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Introduction:
Typhoid fever Rapid Test Device is an immunochromatographic assay designed for the qualitative detection of specific IgG and IgM andodies against a specific Salmonella typhi antigen(1-3) in human serum, plasma or whole blood. It is intended to be used as in vitro diagnostic of typhoid fever. The results obtained should not be the sole determinant for clinical decision.
Principle:
Typhoid fever Rapid Test Device (Whole Blood/Serum/Plasma) is a qualitative membrane-based immunoassay for the detection of specific IgM antibodies against a specific Salmonella typhiantigen in whole blood, serum, or plasma. During testing, the specimen reacts with Goat anti-Human IgM/IgG -coated particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the Salmonella typhiantigen in test line region. If the specimen contains IgM antibodies against a specific Salmonella typhi antigen, a colored line will appear in test line region. If the specimen does not contain IgM antibodies against a specific Salmonella typhiantigen, no colored line will appear in the test line regions, indicating a negative result. To serve as a procedural control, a colored line will always in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.
